The kinetic analysis of γ-aminobutyric acid (GABA) production in buckwheat after high hydrostatic pressure
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Bibliographic record
Abstract
We aimed to investigate the effect of high hydrostatic pressure (HHP) on the production of amino acids, including GABA, in buckwheat during preservation. Buckwheat was soaked in 0–0.5 % glutamic acid solution and exposed to HHP treatment at 200 or 400 MPa. The concentrations of amino acids, except glutamic acid, increased with HHP treatment and preservation. GABA production tended to be higher in a 0.3 g/mL Glu solution under 400 MPa. The relationship between initial glutamate concentration and GABA production rate was bell-shaped, with a maximum approximately 50 µmol/g for the 200-MPa and untreated samples. The Km, Ki, and Vmax were calculated from the Michaelis–Menten equation with substrate inhibition. The Km changed after 400-MPa HHP treatment, whereas Vmax and Ki increased in a pressure-dependent manner. Combining Glu and HHP treatment can produce buckwheat with enhanced GABA production.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.021 |
| Science and technology studies | 0.000 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it