The kinetic analysis of γ-aminobutyric acid (GABA) production in buckwheat after high hydrostatic pressure
Bibliographic record
Abstract
We aimed to investigate the effect of high hydrostatic pressure (HHP) on the production of amino acids, including GABA, in buckwheat during preservation. Buckwheat was soaked in 0–0.5 % glutamic acid solution and exposed to HHP treatment at 200 or 400 MPa. The concentrations of amino acids, except glutamic acid, increased with HHP treatment and preservation. GABA production tended to be higher in a 0.3 g/mL Glu solution under 400 MPa. The relationship between initial glutamate concentration and GABA production rate was bell-shaped, with a maximum approximately 50 µmol/g for the 200-MPa and untreated samples. The Km, Ki, and Vmax were calculated from the Michaelis–Menten equation with substrate inhibition. The Km changed after 400-MPa HHP treatment, whereas Vmax and Ki increased in a pressure-dependent manner. Combining Glu and HHP treatment can produce buckwheat with enhanced GABA production.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".