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Record W4400965089 · doi:10.1101/2024.07.22.604677

Identification of novel loci regulating dormancy in barley and association with hypoxia sensitivity

2024· preprint· en· W4400965089 on OpenAlexafffundabout
Lochlen G. H. Farquharson, Bahram Samanfar, Raja Khanal, Wubishet A. Bekele, Elizabeth K. Brauer

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2024
Typepreprint
Languageen
FieldAgricultural and Biological Sciences
TopicPlant responses to water stress
Canadian institutionsCarleton UniversityUniversity of OttawaAgriculture and Agri-Food Canada
FundersAgriculture and Agri-Food Canada
KeywordsDormancyHypoxia (environmental)Identification (biology)BiologyAssociation (psychology)Sensitivity (control systems)GeneticsBotanyPsychologyChemistryEngineeringGerminationOxygen

Abstract

fetched live from OpenAlex

Abstract Low seed dormancy is an essential trait in malting barley since malting involves rapid and uniform induction of germination. At least two major QTLs on chromosome 5H, SD1 and SD2, regulate dormancy in multiple barley populations, and additional genetic regions are thought to be involved. To identify novel genetic loci that might be associated with dormancy, a panel of genotypes from diverse agro-ecosystems were evaluated alongside two Canadian biparental populations for germination rate. Association mapping revealed QTLs within the SD1 and SD2 loci in the Canadian populations, while neither of these loci were linked to dormancy in the diversity panel. The diversity panel identified 14 additional marker-trait associations, including novel genetic loci. An alanine aminotransferase ( AlaAT1 ) underlies dormancy regulation at the SD1 allele and is thought to help mitigate the suppressive effects of hypoxia on respiration during grain fill. Additional testing with four genotypes carrying either dormant or non-dormant AlaAT1 alleles revealed that dormant alleles had increased hypoxia sensitivity and hypoxia-responsive gene expression during grain fill. Together, this work indicates that multiple genetic regions influence dormancy and suggests that hypoxia influences dormancy establishment in barley. Highlight Dormancy is regulated by distinct genetic regions in North American barley compared to African barley. The SD1 locus influences dormancy in North American lines and genotypes with the dormant SD1 allele are more sensitive to hypoxia.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.025
Threshold uncertainty score0.050

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.195
Teacher spread0.184 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes3
Has abstractyes

Explore more

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