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Record W4401631602 · doi:10.22215/etd/2024-16064

Unmasking the Epigenetic Landscape of Freeze Tolerance in the Grey Tree Frog

2024· dissertation· en· W4401631602 on OpenAlexaff
Saif Ur Rehman

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Molecular Biology Research
Canadian institutionsCarleton University
Fundersnot available
KeywordsEpigeneticsHistoneBiologyAcetylationChromatinHistone H3Histone methyltransferaseLysineHistone methylationHistone codeChromatin remodelingCell biologyHistone H4Translation (biology)Computational biologyGeneticsGene expressionGeneDNA methylationMessenger RNANucleosomeAmino acid

Abstract

fetched live from OpenAlex

For the breadth of the winter, Dryophytes versicolor can survive full body freezing utilizing a phenomenon known as metabolic rate depression (MRD).Differentially expressed miRNA regulate translation through mRNA targets and prove to be a promising candidate for the study of stress tolerance.Additional epigenetic transcriptional control on gene expression, such as histone methylation and acetylation, also implement a balance between permissive and restricted chromatin as required to endure freezing.The current study utilizes next-generation sequencing and bioinformatic analyses to characterize changes in the microRNAome of D. versicolor muscle tissue in response to freezing.Adding to this, the interplay between histone lysine methyl and acetyl transferases (HKMTs, HATs), as well as the abundance of various acetyl-lysine and methyl-lysine moieties on histone H3 and H4 were examined.This study highlights the regulative role played by miRNA and histone modifications on the overall repression of energy-expensive processes during freezing in muscle tissue.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Other · Consensus signal: none
Teacher disagreement score0.001
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.258
Teacher spread0.240 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreOther

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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