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Record W4402186999 · doi:10.32920/26866660

Modulating the Cell Proteome

2024· preprint· en· W4402186999 on OpenAlexaff
Joelle Stilwell

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA Interference and Gene Delivery
Canadian institutionsToronto Metropolitan University
Fundersnot available
KeywordsProteomeComputational biologyCellCell biologyChemistryComputer scienceBiologyBiochemistry

Abstract

fetched live from OpenAlex

Cells exposed to therapeutic ultrasound-microbubbles (USMB) exhibit a variety of bioeffects such as plasma membrane disruption, enhanced endocytosis, leakage, and apoptosis in response to the USMB-induced stress. Environmental stresses can influence the cell proteome, causing changes in protein expression. This study investigates the effect of USMB on the proteome of AML cells using mass spectrometry. USMB induced notable changes in the cell proteome at both 6 and 24 hrs post treatment, with 77 proteins identified. The USMB parameters also influenced the protein expression with the presence of microbubbles and time, having a significant influence on the magnitude of change as well as the proteins affected. Notable proteins include Annexin A1, S100 A10, A11 and heme oxygenase with average fold changes of 2.60 and 2.40, 1.52 and 1.76, 1.27 and 1.40 and 4.45 and 1.27 at 6 and 24 hrs respectively, post USMB treatment. In addition, the proteins influenced play roles in known cellular responses to the USMB treatment as well as mechanical and oxidative stresses.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.250
Teacher spread0.237 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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