Abstract A044 Enhanced disease detection using single cell RNAseq in children with brain cancer
Bibliographic record
Abstract
Abstract Introduction: Serial liquid biopsy sampling is used to risk-stratify and assign therapy in childhood acute lymphoblastic leukemia. While circulating tumor DNA (ctDNA) from cerebrospinal fluid (CSF) has been evaluated, experience with single cell RNA sequencing (scRNAseq) and circulating tumor cells (CTCs) in childhood brain cancer has not been well described. Furthermore, a large scale analysis of the cellular ecosystem using scRNAseq in CSF has not been performed in this population. Here we assessed the value of high throughput scRNAseq on CSF samples from children with brain cancer, to test the hypothesis that CTCs from CSF could provide insight into diagnosis and disease progression. Method: We profiled cells in the CSF of both high and standard risk brain cancer patients collected at Sydney Children’s Hospital, Randwick, Australia. By using advanced informatic tools, we assessed cell counts, gene expression, copy number variation, single nucleotide variation, cell-cell interactions and performed pathway analysis. These data were evaluated alongside clinical disease parameters including CSF cytology and matched tumour bulk sequencing data (RNA and DNA) obtained through the ZERO Childhood Cancer Precision Medicine Program. Results: scRNAseq was performed on 92,965 CSF cells from 22 samples across 14 patients (including 4 patients with sequential samples). The cohort includes ATRT, DLGNT, DMG, medulloblastoma, infant-type hemispheric glioma, astrocytoma, pineoblastoma, and ependymoma. Cells identified were multiple immune subsets, fibroblasts, microglial and cancer cells. Analysis of 10 timepoints where scRNAseq was matched to cytology showed both approaches found positive disease (>10 putative cancer cells) in 2 timepoints and negative disease in 5 timepoints. Our scRNAseq approach found disease in 2 timepoints that were negative and 1 which was uncertain by cytology. Matching results to bulk RNA sequencing from the primary tumour showed that, in samples containing high CTC numbers (n= 3), we were able to confidently identify patient specific biomarkers of disease (including MYC, PVT1, and OTX2) and have identified evidence of CNS disease heterogeneity. In addition, we identified cell-cell interactions in a patient with DMG. These interactions were via IGTB3, which has previously been associated with metastasis. Finally, serial samples have been collected to enable minimally invasive disease monitoring, including in one patient with ATRT who is currently disease free. Conclusion: We show that scRNAseq of CTCs is feasible in pediatric brain tumors. This provides a novel method to understand the biology of pediatric brain cancer. By coupling high sensitivity detection with a detailed characterization of individual cells, we have shown potential utility in disease monitoring including at the level of minimal residual disease. In future, this technology could be applied to assess treatment response kinetics, aid treatment selection, enable detection of early relapse and assist in disease prognostication. Citation Format: Robert Salomon, Wenyan Li, Mojgan Toumari, Aileen Lowe, Chelsea Mayoh, Paulettte Barahona, Loretta MS Lau, Jordan Staunton, Erica Jacobson, Sumanth Nagabushan, Neevika Manoharan, Ruth Mitchell, Faustine Ong, Michelle Haber, David S Ziegler, Mark J. Cowley, Marion K. Mateos. Enhanced disease detection using single cell RNAseq in children with brain cancer [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Advances in Pediatric Cancer Research; 2024 Sep 5-8; Toronto, Ontario, Canada. Philadelphia (PA): AACR; Cancer Res 2024;84(17 Suppl):Abstract nr A044.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".