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Record W4402285586 · doi:10.1101/2024.09.04.611284

Proteomic Investigation of Neurotrophic <i>trans</i> -Banglene Reveals Potential Link to Iron Homeostasis

2024· preprint· en· W4402285586 on OpenAlexaff
Piyumi B. Wijesiri Gunawardana, Khyati Gohil, Kyung‐Mee Moon, Leonard J. Foster, Florence Williams

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2024
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCellular transport and secretion
Canadian institutionsUniversity of British ColumbiaUniversity of Alberta
Fundersnot available
KeywordsIron homeostasisHomeostasisLink (geometry)NeurotrophinCell biologyNeurotrophic factorsChemistryNeuroscienceBiologyBiochemistryComputer scienceMetabolismReceptor

Abstract

fetched live from OpenAlex

ABSTRACT In an effort to gain insight into cellular systems impacted by neurotrophic trans -banglene ( t- BG), global proteomic profiling and Western blot analyses were employed. Expression level changes in response to t -BG treatment were compared to those observed with nerve growth factor (NGF), a natural neurotrophic protein and functional analog to t -BG. Findings from these studies did not point to direct interception of NGF/TrkA signaling by t- BG. Instead, significant alterations in iron-binding and iron-regulating proteins were observed. Intracellular iron measurements by FerroOrange indicate lower ferrous (Fe 2+ ) iron levels in t -BG treated cells but not in NGF treated cells. These results highlight a potential connection between iron regulation and neurotrophic activity.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.200
Teacher spread0.191 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2024
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)→Same topicCellular transport and secretion→French-language works237,207→