Opuntia ficus indica cladode extract inhibit DNA double-strand breaks and locally multiply damaged sites induced by gamma radiation
Bibliographic record
Abstract
It is beyond doubt that radiotherapy is extremely effective in treating a wide variety of cancers. The sensitivity of the surrounding normal tissues limits the amount of radiation administered to the tumor. There is an urgent need to develop a treatment that combines pharmacological treatment with ionizing radiation (IR) specifically designed to specifically target cancer cells while protecting the surrounding normal tissue, resulting in an increase in the efficacy of the cancer treatment. IR could cause many types of DNA lesions. Double-strand breaks (DSBs) andlocally multiple damaged sites (LMDS)arethe main radiotoxic damages.Recently, the identification of new antioxidants from natural sources has attracted the attention of scientists. In this context, the present study aims to determine if the Opuntia ficus indica cladode extract (CE) can be used as a radioprotector. MATERIALS AND METHODS: Cs γ-radiation (25-700 Gy) in the absence or presence of cactus cladode extract (CCE) was added to theE. colibase excision repair. The amounts of both DNA damages were calculated using the electrophoretic method. RESULTS: The irradiation of DNA in the presence of CCE induced a dramatic decrease of the yields of purine and pyrimidine-DSB. A decrease of65 % and 84 % of the purine and pyrimidine-DSB sensitive sites have been calculated, respectively, when the sample added CCE3 during the radiotreatment. Moreover, a reduction of 80 % in the amount of Nth + Fpg-DSB SSs (non-DSB cluster damage) after γ-irradiation in the presence of CCE3 was observed. CONCLUSION: Through the present it was found that the CCE can play an important role as a radio protector, maybe by scavenging the ROS formed during radio treatment or by other unknown pathways. The most toxic DNA lesions (DSBs, and LMDS) decreased dramatically. Studies aimed at obtaining more documentation about CCE components with potential radio-preventive activity are desirable because of their protective properties.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".