Development and evaluation of a monoclonal antibody-based competitive ELISA for detecting porcine deltacoronavirus antibodies
Bibliographic record
Abstract
Abstract Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus that can cause acute diarrhea and vomiting in newborn piglets and poses a potential risk for cross-species transmission. It is necessary to develop an effective serological diagnostic tool for the surveillance of PDCoV infection and vaccine immunity effects. In this study, we developed a monoclonal antibody-based competitive ELISA (cELISA) that selected the purified recombinant PDCoV nucleocapsid (N) protein as the coating antigen to detect PDCoV antibodies. To evaluate the diagnostic performance of the cELISA, 122 swine serum samples (39 positive and 83 negative) were tested and the results were compared with an indirect immunofluorescence assay (IFA) as the reference method. By receiver operating characteristic (ROC) curve analysis, the optimum cutoff value of percent inhibition (PI) was determined to be 26.8%, which showed excellent diagnostic performance, with an area under the curve (AUC) of 0.9919, a diagnostic sensitivity of 97.44% and a diagnostic specificity of 96.34%. Furthermore, there was good agreement between the cELISA and virus neutralization test (VNT) for the detection of PDCoV antibodies, with a coincidence rate of 92.7%, and the κ analysis showed almost perfect agreement (κ = 0.851). Overall, the established cELISA showed good diagnostic performance, including sensitivity, specificity and repeatability, and can be used for diagnostic assistance, evaluating the response to vaccination and assessing swine herd immunity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.002 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".