Tomato cystatin SlCYS8 as a trigger of drought tolerance and tuber yield in potato
Bibliographic record
Abstract
Abstract Current climate change scenarios predict an increased incidence of drought episodes likely to affect potato crops worldwide. Potato exhibits a low-density, shallow root system that makes it particularly vulnerable to water shortage and any successful attempt to implement drought tolerance in cultivated potato varieties is potentially relevant from an agronomic standpoint. In this study, we assessed the potential of tomato cystatin SlCYS8 to promote drought tolerance in SlCYS8-expressing potato lines by induction of stress-related pleiotropy. Up to now, protease inhibitors of the cystatin protein superfamily have been mostly considered as biotechnological tools to engineer pest or pathogen resistance in crops, but several recent studies have also revealed a possible link between abiotic stress tolerance and these regulatory proteins in leaf tissue. SlCYS8-expressing plantlets grown on culture medium containing the drought mimic polyethylene glycol (PEG) exhibited an elevated root-to-shoot ratio, an indicator of drought tolerance in potato. A similar conclusion could be drawn with greenhouse-grown acclimated plants, confirming a relative root growth-promoting effect for the recombinant inhibitor upon water deficit. SlCYS8-potato lines also showed a high tuber yield compared to the control line under both limiting and non-limiting water regimes, suggesting an improved efficiency of the primary metabolism and the avoidance of a growth– stress response tradeoff in the modified lines. Accordingly, SlCYS8 expression was associated with a stress response-oriented proteome in leaves likely explained by pleiotropic effects of the recombinant cystatin driving the constitutive expression of stress-related proteins and the upregulation of primary metabolism-associated proteins. Overall, these data suggest the potential of cystatins as molecular triggers of tuber biomass production and drought resilience in potato. Complementary studies will be warranted to assess tuber yield of the SlCYS8-lines under different water regimes in field conditions.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".