Conditional Caspase Expression in Zebrafish Ovaries Induces Sterility
Bibliographic record
Abstract
Biocontainment methods for aquaculture are in development and implementation to prevent farmed fish from breeding and thus invading the surrounding environment following an escape from open netpens. Current methods have not demonstrated 100% efficacy in achieving the desired sterility, highlighting the need for the development of novel biocontainment strategies to implement in aquaculture. Using zebrafish as a model organism, we determined the efficacy of the Cre‐LoxP system to conditionally express the active form of caspase‐3, an apoptosis‐inducing cell‐death protein, in the developing ovary. This was achieved through the use of two transgenic lines, Tg ( zpc:cre; cmlc2-mCherry ) and Tg ( ef1a:loxP:caspase3:loxP; cmlc2-EGFP ), that induce the expression of active caspases in the developing ovary of progeny containing both constructs. Fish positive for only one of the two constructs displayed wildtype (WT) gonadal tissue and bred successfully. However, 78.3% of progeny that carried both constructs did not breed successfully and possessed either little or no gonadal tissue compared to WT controls upon dissection. When combined with induced triploidy, fish that were transgenic for both constructs were 100%. Our results suggest that conditional expression of a cell‐death‐inducing protein could be used as part of a strategy for the confinement of fish species and demonstrates the utility of “stacking” containment approaches to enhance reproductive containment.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".