Cloning, sequence analysis, and functional study of the PeDof-4 gene in response to drought stress
Bibliographic record
Abstract
DNA-binding with one finger (Dof) was a functionally important gene in plant stress tolerance processes. The cloned full-length (849bp) passion fruit PeDof-4 gene was sequenced, which encodes 282 amino acids with the molecular formula C1274H1983N375O408S14, molecular weight 29529.87 u, and theoretical isoelectric point pI of 8.96. Evolutionary tree analysis showed that the PeDof-4-encoded proteins had a high degree of identity with Dof-encoded proteins in other plants. Blastp of the PeDof-4 encoded proteins revealed a closer affinity to the proteins encoded by the Dof of Theobroma cacao. The relative expression of the PeDof-4 gene was higher at 50% of the relative soil moisture content in the fluorescence qPCR experiments under different levels of drought stress. Tissue-specific analysis showed that the highest expression of this gene was found in the roots. Overexpression of GUS gene under the PeDof-4 promoter inbidopsis thaliana showed that PeDof-4 could significantly respond to drought stress. Bangladesh J. Bot. 52(3): 653-663, 2024 (September) Special
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".