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Late Breaking Abstract - Developing a prognostic SLB-derived gene signature in fibrosing ILDs

2024· article· en· W4404104108 on OpenAlexaff
Seung‐Jun Kim, Nathashi Jayawardena, Matthew J. Cecchini, Keith Kwan, William W. Dawson, Daniel T. Passos, Frederick A. Dick, Marco Mura

Bibliographic record

Venuenot available
Typearticle
Languageen
FieldMedicine
TopicEosinophilic Disorders and Syndromes
Canadian institutionsWestern UniversityLondon Health Sciences Centre
Fundersnot available
KeywordsSignature (topology)Gene signatureComputer scienceGeneBiologyGeneticsMathematicsGene expression

Abstract

fetched live from OpenAlex

Persisting diagnostic uncertainty in ~20% of cases who undergo surgical lung biopsies (SLBs), and significant variability in clinical courses and outcomes for every given subtype of fibrosing ILD are challenges in managing fibrosing interstitial lung diseases (ILDs). In this registered, prospective cohort study ( NCT03836417 ), treatment-naive patients diagnosed with fibrosing ILD based on SLBs and multi-disciplinary discussion, and > 1 year of follow-up were prospectively followed after SLB. The primary endpoint was progression of disease, as defined by the ATS guidelines. RNA-seq was performed on fresh-frozen tissue obtained from the operating room of every SLB. Between June 2019 and June 2023, 44 patients were included in the study. During this period, 14 (32%) progression of disease events were observed, including 7 deaths and 4 lung transplants. Clinical variables were considered (age, gender, BMI, diagnosis of IPF, baseline FVC, DLCO and 6MWD), but only BMI was significantly protective against progression. After variance filtering, we divided our cohort into half to perform training and validation, and resampled 100 times. Penalized Lasso Cox regression was used with leave-one-out cross validation to select genes associated with progression. The median AUC and Harrell’s C-index were 0.777 (SD = 0.09) and 0.743 (SD= 0.13), respectively. The integrated Brier score was 0.158. HLA-B expression predicted progression, while HLA-DQA1, RP11-532E4.2 and TSIX expression was protective in >30 % of resampling. We identified a gene signature obtained from SLBs that predicts progression in fibrosing ILDs at the time of biopsy, agnostic to ILD subtype. We plan to perform an external validation of our findings.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.004
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.011

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0020.004
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.001
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.280
Teacher spread0.261 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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