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Record W4404534526 · doi:10.1101/2024.11.18.624148

Whole-Genome Sequencing of the Wild Barley Diversity Collection: A Resource for Identifying and Exploiting Genetic Variation for Cultivated Barley Improvement

2024· preprint· en· W4404534526 on OpenAlexaff
Ahmad H. Sallam, Yu Guo, Murukarthick Jayakodi, Axel Himmelbach, Anne Fiebig, Jamie Simmons, Gerit Bethke, Y. C. Lee, Rebecca Spanner, Ana Badea, Michaël Baum, François Belzile, Roi Ben‐David, Robert Brueggeman, Austin J. Case, Luigi Cattivelli, Michael H. Davis, Christoph Dockter, Jaroslav Doležel, Antonín Dreiseitl, Ryan Gavin, Lior Glick, Stephan Greiner, Ruth Hamilton, Patrick M. Hayes, Scott Heisel, Cynthia A. Henson, Benjamin Kilian, Takao Komatsuda, Chengdao Li, Cheng Liu, Ramamurthy Mahalingam, Maren Maruschewski, Oadi Matny, Andreas Maurer, Klaus Mayer, Itay Mayrose, Peter L. Morrell, Matthew Moscou, Gary J. Muehlbauer, Youko Oono, Frank Ordon, Hakan Özkan, Aleš Pečinka, Dragan Perović, Klaus Pillen, Mohammad Pourkheirandish, Joanne Russell, Jan Šafář, Silvio Salvi, Miguel Sanchez‐Garcia, Kazuhiro Sato, Thomas Schmutzer, Uwe Scholz, Jeness C. Scott, Gurcharn S. Brar, Kevin P. Smith, Mark E. Sorrells, M. Spannagl, Nils Stein, Alessandro Tondelli, Roberto Tuberosa, James R. Tucker, T. Kelly Turkington, J. Valkoun, Ramesh Pal Singh Verma, Marcus A. Vinje, Maria von Korff Schmising, Jason G. Walling, Robbie Waugh, Roger P. Wise, Brande B. H. Wulff, Shengming Yang, Guoping Zhang, Martin Mascher, Brian J. Steffenson

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2024
Typepreprint
Languageen
FieldAgricultural and Biological Sciences
TopicWheat and Barley Genetics and Pathology
Canadian institutionsUniversity of AlbertaUniversité LavalAgriculture and Agri-Food Canada
Fundersnot available
KeywordsBiologyGeneticsHordeum vulgareLocus (genetics)GenomeGenetic diversityWhole genome sequencingQuantitative trait locusSingle-nucleotide polymorphismChromosomeStem rustGeneGenotypeBotanyPoaceaePopulation

Abstract

fetched live from OpenAlex

Abstract To exploit allelic variation in Hordeum vulgare subsp. spontaneum , the Wild Barley Diversity Collection was evaluated for several agronomic traits and subjected to paired-end Illumina sequencing at ∼9X depth, generating 109.5 million single nucleotide polymorphisms after alignment to the Morex V3 assembly. A genome-wide association study of lemma color identified one marker-trait association (MTA) on chromosome 1HL close to HvBlp , the cloned gene controlling black lemma. Four MTAs were identified for stem rust resistance: one co-locating to the complex RMRL1-RMRL2 locus on 5HL, and three novel loci on 1HS, 1HL, and 5HL. Six MTAs for days to heading (DTH) on vernalized plants were identified on all chromosomes except 1H and 6H. Two MTAs for DTH on non-vernalized plants were identified on chromosomes 1HL and 2HS. All MTAs for DTH were novel. The whole genome sequence data described herein will facilitate the identification and utilization of new alleles for barley improvement.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.011
Threshold uncertainty score0.022

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0020.003
Science and technology studies0.0010.000
Scholarly communication0.0010.000
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0050.004

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.030
GPT teacher head0.214
Teacher spread0.183 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2024
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)→Same topicWheat and Barley Genetics and Pathology→French-language works237,207→