Overwintering temperature affects lipid and fatty acid metabolism in hepatopancreas and ovary of female mud crab Scylla paramamosain
Bibliographic record
Abstract
The influence of four overwintering temperatures, namely 8 ℃, 12 ℃, 16 ℃, and 20 ℃, on lipid metabolism and fatty acid profile in the hepatopancreas and ovary tissues of female mud crab Scylla paramamosain was investigated during eight weeks. According to the results, the hepatosomatic index (HSI) of the crabs after overwintering at 16 ℃ and 20 ℃ was significantly lower than that pre-overwintering ( P < 0.05), however, overwintering did not affect the gonadosomatic index (GSI) ( P > 0.05). Moreover, the increased overwintering temperature significantly decreased the triglyceride (TG) content in the hepatopancreas ( P < 0.05), while the ovary tissue remained unaffected ( P > 0.05). Hemolymph biochemical indices demonstrated that the level of low-density lipoprotein cholesterol (LDL-C) increased markedly as temperature rose ( P < 0.05). In addition, the fatty acid profile of hepatopancreas tissue did not change significantly at different overwintering temperatures ( P > 0.05), however, the content of EPA in ovary tissue significantly increased with the temperature rise ( P < 0.05). Furthermore, the rise in temperature significantly upregulated the expressions of lipid catabolism genes ( hsl , cpt Ⅰ ) and lipid transport genes ( fabp3 , fatp4 ), while significantly downregula t ed the expressions of lipid synthesis genes ( acc , g6pd ) in the hepatopancreas. Meanwhile, the temperature did not affect the expressions of lipid synthesis genes ( acc , fas , g6pd ) and lipid catabolism genes ( hsl , aco , cpt Ⅰ , cpt Ⅱ ) in ovary tissue. Still, the temperature increase significantly upregulated the expressions of PUFA synthesis genes ( Δ6fad , elovl4 ). In conclusion, overwintering at 16 ℃ and 20 ℃ promoted the process of lipid catabolism and transport in hepatopancreas tissue of mud crabs. However, overwintering at 20 ℃ was beneficial for accumulating more EPA in ovary tissue from a breeding perspective. • Overwintering temperature affected HSI but not GSI in Scylla paramamosain . • Overwintering at 16°C and 20°C enhanced hepatopancreatic lipid catabolism and transport while decreasing lipid synthesis. • Overwintering temperature had no impact on ovarian lipid synthesis and catabolism. • Ovary tissue promoted LC-PUFA synthesis and accumulated more EPA at 20℃.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".