BmE2F1 regulates endoreplication of silk gland cells in silkworm, Bombyx mori
Bibliographic record
Abstract
Endoreplication is particularly important in the context of silk protein synthesis within the silk gland cells of silkworms. Our previous research indicated that the BmE2F1 enhances the silk yield of silkworm cocoons, but the underlying molecular mechanism remains elusive. In this study, we employed RNA-sequencing to dissect the transcriptional profiles of silk glands in the wild-type Dazao silkworm strain and the overexpression (OE) silkworm strain with specific overexpression of the BmE2F1 gene in silk glands. Among the 1126 differentially expressed genes (DEGs), many related to DNA replication (endoreplication in silk glands of silkworm larvae) were significantly enriched. Quantitative real-time PCR confirmed that overexpression of BmE2F1 led to a substantial increase in the expression of 13 genes involved in the DNA replication pathway. Additionally, BmE2F1 upregulated the expression of BmCyclin E , a pivotal gene in the G/S phase transition. Moreover, BmE2F1 overexpression in silk glands significantly boosted DNA replication and concurrently increased the DNA content within silk glands. In conclusion, BmE2F1 regulates endoreplication in silk gland cells of silkworms through dual mechanisms: firstly, by enhancing the formation of the DNA replication complex; and secondly, by facilitating the cells' entry into the S phase. • A novel gene, BmE2F1 , regulates endoreplication in silk gland cells of silkworm. • BmE2F1 functions as a transcriptional activator of cell-cycle related genes and DNA replication genes in silk gland cells. • BmE2F1 modulates endoreplication in silk gland cells to enhance silk yields in silkworm.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".