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Record W4405559960 · doi:10.21769/bioprotoc.5177

Confocal Live Imaging of Reproductive Organs Development in <em>Arabidopsis</em>

2024· article· en· W4405559960 on OpenAlexafffund
Binghan Wang, Amélie Bauer, Andrea Gómez‐Felipe, Sylvia Rodrigues da Silveira, Daniel Kierzkowski

Bibliographic record

VenueBIO-PROTOCOL · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant Reproductive Biology
Canadian institutionsUniversité de MontréalUniversité de Sherbrooke
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsArabidopsisConfocalBiologyCell biologyConfocal microscopyLive cell imagingOpticsPhysicsGeneticsGeneCell

Abstract

fetched live from OpenAlex

including both the stamen and gynoecium. We also discuss how to acquire high-quality images suitable for efficient 2D and 3D segmentation that allow the quantification of cellular dynamics underlying their development. Key features • Fine dissection of tiny and tightly enclosed floral organs. • Confocal live imaging method allowing long-term observation of plant reproductive morphogenesis. • Assessing the quality of acquired images for efficient segmentation at cellular resolution in 2D and 3D.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.326
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.278
Teacher spread0.265 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations5
Published2024
Admission routes2
Has abstractyes

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