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Record W4405660035 · doi:10.1016/j.fishres.2024.107254

Fish ID face-off: A comparison of genetic barcoding and otolith shape analysis for streamlining species identification of mesopelagic fishes

2024· article· en· W4405660035 on OpenAlexafffund
L. Walton, Micah Quindazzi, Stéphane Gauthier, Catherine Stevens

Bibliographic record

VenueFisheries Research · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicIdentification and Quantification in Food
Canadian institutionsFisheries and Oceans CanadaUniversity of Victoria
FundersCanada First Research Excellence FundUniversity of VictoriaUniversity of Guelph
KeywordsMesopelagic zoneOtolithDNA barcodingFish <Actinopterygii>FisheryIdentification (biology)BiologyZoologyEcologyPelagic zone

Abstract

fetched live from OpenAlex

Identifying fish to species level is important for fisheries research as it ensures the accuracy of catch data reported by fishing vessels and informs best management strategies for harvested taxa. Genetic methods are one of the most common techniques used for identifying fish species but can be time-consuming and costly and may lead to incorrect or incomplete identifications if genetic baselines do not exist. Visual identifications using otolith shape are an inexpensive alternative that can be used to identify large numbers of fish samples quickly and with high accuracy. The objective of this study was to compare two methods of taxonomic identification ( i.e. , DNA barcoding of the COI-5P marker and visual identifications using body morphology, distinguishing features and otolith shape) for 240 specimens of mesopelagic fishes as they occupy a critical role in marine food webs. We also tested the effectiveness of geometric morphometrics in delineating species of mesopelagic fishes based on otolith shape. Our results showed that visual identifications agreed with genetic identifications 89 % of the time, and that both techniques were effective for identifying mesopelagic fishes. Additionally, we found that geometric morphometrics were successful in distinguishing mesopelagic fishes using otolith shape 86 % of the time. Otolith shape is a useful tool for the taxonomic identification of mesopelagic fishes in the Northeast Pacific Ocean and should be employed with higher frequency by fisheries researchers, especially in the absence of taxonomic or genetic expertise.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.197
Threshold uncertainty score0.444

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.117
GPT teacher head0.395
Teacher spread0.278 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations6
Published2024
Admission routes2
Has abstractyes

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