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Dynamic pH fluctuations in cancer cells on CMOS-based Lab-on-Chip ISFET arrays

2024· article· en· W4405709337 on OpenAlexfundno aff
Melina Beykou, Costanza Gulli, Vicky Bousgouni, Nicolas Moser, Chris Bakal, Pantelis Georgiou

Bibliographic record

Venuenot available
Typearticle
Languageen
FieldChemical Engineering
TopicAnalytical Chemistry and Sensors
Canadian institutionsnot available
FundersInstitute of Cancer ResearchImperial College London
KeywordsISFETLab-on-a-chipCMOSChipComputer scienceOptoelectronicsNanotechnologyMaterials scienceSystem on a chipElectronic engineeringElectrical engineeringMicrofluidicsEngineeringTransistorEmbedded systemField-effect transistorVoltage

Abstract

fetched live from OpenAlex

Early diagnosis of cancer is a key avenue to improving cancer incidence rates and prognosis. This paper presents a rapid assay for live, mammalian cancer cell culture on a Lab-on-Chip ISFET array fabricated in CMOS technology. Detection of osteosarcoma cells is recorded as a significant difference in endpoint $\mathbf{p H}$ on ISFET sensors. Control measurements of $\mathbf{p H}$ change in cell culture media due to any CO2supply fluctuations in an incubator allow compensation of non-ideal effects including sensor drift and baseline $\mathbf{p H}$ change associated with a bicarbonate buffering system. Distinguishing metrics, including total drift rate and standard deviation of sensor output are extracted and attributed to cell presence on-chip. Mean total drift rate shows an 89.7% difference between sample and control wells, aiding characterisation of cancer cell presence. Standard deviation captures the noise output attributed to cell culture on the sensor surface, reporting an $\mathbf{8 1. 4 \%}$ difference between populations. This Point-of-Care diagnostic device and assay for rapid cell detection on a Lab-on-Chip shows significant potential to provide a rapid and cost-effective diagnostic tool for live cell measurements.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.265
Teacher spread0.253 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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