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Storage and Stocking of Anaerobes

2023· other· en· W4406352353 on OpenAlexaff
Frances Valencia‐Shelton, Kristen L. Brown

Bibliographic record

VenueClinMicroNow · 2023
Typeother
Languageen
FieldMedicine
TopicSpaceflight effects on biology
Canadian institutionsUniversity of Calgary
Fundersnot available
KeywordsVialCryopreservationPolypropyleneEnvironmental scienceBiologyChemistryMaterials scienceChromatographyComposite material

Abstract

fetched live from OpenAlex

Abstract Preservation of anaerobic isolates may be required for many reasons, including clinical inquiry, research, teaching, and quality assurance testing. The method of storage used will depend on the length of storage required. Short‐term storage may occur using routine culture media on the benchtop or frozen, though self‐defrosting units must be avoided to prevent repeated freeze‐thaw of the stored isolates. Glass vials may be used and are the preferred method for years of long‐term storage. One of the most used vial types consists of plastic polypropylene tubes with screw tops. Commercially preprepared solutions for cryopreservation in vials are available, some of which include beads onto which the organisms can adhere, which can make for an easier job of thawing and culturing frozen stores. Products should indicate that they are for the frozen maintenance of bacterial cultures, but may not specifically reference anaerobes. A heavy inoculum is recommended to ensure some cell survival throughout the freeze‐thaw cycle.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.106
Threshold uncertainty score0.624

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.300
Teacher spread0.285 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreOther

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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