Multi-omics analysis provides new insights into the molecular mechanisms underlying colostral immunoglobulin G absorption in the gut of neonatal goat kids
Why this work is in the frame
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Bibliographic record
Abstract
Early colostrum feeding facilitates the passive transfer of immunoglobulin G (IgG), which contributes to the defensive establishment of neonates; however, the molecular mechanisms of IgG absorption in the small intestine of neonatal mammals remain largely unknown. In this study, a total of 16 neonatal goat kids with similar body weight (2.05 ± 0.31 kg) were selected and randomly assigned to 1 of 2 feeding treatments: normal colostrum feeding (NCF, n = 8) or delayed colostrum feeding (DCF, n = 8). Multi-omics coupled with individual bioinformatics analyses were employed to obtain a comprehensive understanding of the molecular mechanisms of IgG absorption. Phenotypic analysis showed that the capacity of IgG absorption was largely affected ( P < 0.05) by colostrum feeding time in neonatal goat kids. Weighted gene co-expression network analysis generated 23 gene modules (gene module defined M1 to M23) and the M12 module was highly correlated (|r| > 0.70 and adjusted P < 0.01) with IgG absorption. Genes in M12 were involved in the endocytosis pathway, especially related to clathrin-mediated endocytosis and macropinocytosis. The differentially expressed genes (DEGs) enriched in the above-mentioned pathways regulated the clathrin synthesis ( CLTC ), the formation of clathrin-coated vesicles ( ARPC1A ), and the sorting and recycling endosomes ( CAPZA2 , KIAA0196 , RAB10 , RAB11A and VPS35 ) as well as the formation of macropinosomes ( FGFR4 and RhoA ) in micropinocytosis, which induced differences in serum IgG concentrations. Additionally, 5 differentially expressed miRNAs (miR-2755-3p, miR-10400-5p, miR-71-5p, miR-2944-3p and miR-2411-3p) were predicted to regulate mRNA involved in clathrin-coated vesicles, Fc receptor for IgG (FcRn)-IgG sorting, and macropinosomes formation that may cause the difference in IgG absorption ability. This study provides new insights into the molecular mechanisms controlling IgG absorption of neonatal ruminants and reveals novel mRNA and miRNA markers involved in clathrin-mediated endocytosis and macropinocytosis which may provide the fundamental knowledge related to IgG absorption to support further study in other mammals.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it