Antioxidant Activity of Hempseed-Infused Kombucha: An In Vivo Study Using <i>Caenorhabditis elegans</i>
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Bibliographic record
Abstract
High Resolution Image Download MS PowerPoint Slide Oxidative stress contributes to aging and degenerative diseases. Hempseed-added kombucha (HK) contains higher levels of antioxidants, phenolics, and proteins than traditional kombucha (CK), suggesting a greater potential to combat oxidative stress. The aim of this study was to evaluate the effect of HK on the increase in resistance to oxidative stress using the in vivo Caenorhabditis elegans biological model. Antioxidant capacity (AC) was measured with DPPH+ and ABTS+ assays, while oxidative stress resistance was assessed via survival and ROS production in C. elegans . The DPPH+ assay showed AC of 0.119 mg Trolox equivalent (TE)/mL for CK and 0.163 mg TE/mL for HK. The ABTS+ assay showed AC of 0.101 mg TE/mL for CK and 0.136 mg TE/mL for HK, with significant differences ( p < 0.05). Juglone-induced oxidative damage was reduced by 10–15% with CK and 30–35% with HK. HK enhanced survival and reduced ROS levels in nematodes. These findings highlight HK’s potential as a superior functional beverage for dietary interventions against oxidative stress.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.002 | 0.005 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it