A59 IMPACT OF ISOVALERATE SUPPLEMENTATION ON MURINE ANTIBODY RESPONSES DURING ENTERIC HELMINTH INFECTION
Bibliographic record
Abstract
Abstract Background Parasitic worm (helminth) infections significantly contribute to human morbidity, with nearly one fifth of the global population chronically infected. Our lab has shown that levels of the short chain fatty acid isovalerate are increased during a murine intestinal helminth infection and that isovalerate supplementation increases helminth fecundity, implying increased worm fitness. Aims Our research aims to elucidate the potential immunomodulatory impact of isovalerate during enteric helminth infection. Methods Using the small intestinal-colonizing mouse roundworm, Heligmosomoides polygyrus (Hp), to model enteric helminth infections, we examined the influence of isovalerate supplementation in the drinking water on total and Hp antigen-specific antibody responses during primary (1o) & secondary (2o) Hp infections in both sexes of C57BL/6J mice. Circulating and mucosal antibody responses were analyzed using enzyme-linked immunosorbent assays and the impact of isovalerate supplementation on lymphocyte populations was assessed in the gut-draining lymph nodes, the spleen, and Peyer’s Patches using flow cytometry. Results Data from one experimental repeat shows a trending reduction in total systemic and total mucosal IgA concentrations in 1oHp infected mice that were supplemented with isovalerate, compared to mice on control drinking water. In mice supplemented with isovalerate in the 2oHp infection model, one experimental repeat shows that isovalerate supplementation does not impact susceptibility to challenge infection or elicit changes to circulating or mucosal antibody levels. Conclusions Preliminary data suggest that isovalerate supplementation may impact total systemic and mucosal antibody concentrations during a 1oHp infection. Ongoing work includes solidifying our preliminary findings with experimental repeats. Future work will assess the impact of isovalerate supplementation on B cell development and antibody concentrations at steady state as well as during infection and elucidate the signalling mechanisms of isovalerate in vivo and in vitro. Furthering our understanding of how isovalerate affects the antibody response during a helminth infection may reveal novel immunomodulatory mechanisms in the intestine and may be applicable to other gut-restricted diseases. Funding Agencies CIHRTRIANGLE
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".