A153 IL10-PRODUCING GUT-RESIDENT TR1 CELLS ARE FOUND AT HIGH FREQUENCIES IN HEALTHY HUMANS AND MICE AND MAY PROTECT AGAINST MURINE ACUTE COLITIS
Bibliographic record
Abstract
Abstract Background Inflammatory bowel disease (IBD) affects an estimated 270,000 people in Canada and is increasing in prevalence. Current treatments, such as anti-TNFα mAbs, block inflammatory pathways, but require repeated dosing and do not reverse intestinal fibrosis. Additionally, a significant subset of IBD patients have relapsing disease and do not respond to biologics. We have shown type 1 regulatory cells (Tr1s) are capable of suppressing inflammation via IL-10, promote gut-healing and barrier function via IL-22, and are long-lasting in mouse models. Aims We aimed to evaluate adoptive transfer of Tr1 cells as a therapy for IBD. We hypothesized that Tr1 cells can prevent inflammatory damage and fibrosis in the context of IBD. Methods The frequency of non-FOXP3 (Tr1) regulatory cells were analyzed from IL-10-eGFP mice and human gut samples from the Australian Donation and Transplantation Biobank to determine the major source of gut-resident T-cell-derived IL-10. Adoptive transfer experiments were performed using 9-10 week old C57BL/6 mice, where mice were briefly irradiated, injected with 1x106 FOXP3neg IL-10+ (Tr1) cells or PBS prior to administration of dextran sulfate sodium (DSS) to induce acute colitis. Weight and disease score were measured for 14 days, followed by histologic analysis of gut tissue and flow cytometric analysis of adoptively transferred (Thy1.1+) and endogenous (Thy1.2+) T cells. Results Tr1 cells act as the major source of intestinal CD4+ gut-resident derived IL-10, as compared to FOXP3+ T regulatory cells in mice and humans (p=0.0051, 0.0156). Further, mice receiving Tr1 adoptive transfer therapy have decreased weight loss at day 7 post DSS administration (p = 0.0163), increased colon length (p=0.0406), decreased colon thickening (p=0.0234), and decreased blinded histologic scoring (p=0.0229) at experimental endpoint. Furthermore, successfully engrafted adoptively transferred Tr1 cells have increased expression of T regulatory marker ICOS in the blood, spleen, and mesenteric lymph node of mice receiving cellular therapy, as compared to endogenous CD4+ T cells (p=0.0003, 0.003, <0.0001). Preliminary experiments suggest human CD4+ ICOS+ T cells secrete high levels of IL-10. Conclusions Tr1 cells are the major T cell producers of anti-inflammatory cytokine IL-10 in both human and murine intestine, producing more IL-10 than FOXP3+ regulatory T cells. Adoptive transfer of Tr1 cells into a murine model of acute colitis protects against weight loss and colon remodeling. Further, these cells are long-lived and detectable up to 8 weeks post transfer. Together, this data suggests Tr1 cellular therapy may be a promising treatment for IBD. Funding Agencies CIHR
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".