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Record W4407626286 · doi:10.1016/j.psj.2025.104908

A Bacillus subtilis xylanase improves nutrient digestibility, intestinal health and growth performance of broiler chickens undergoing an intestinal challenge

2025· article· en· W4407626286 on OpenAlexaff
Catarina Stefanello, Yuri Katagiri Dalmoro, Heitor Vieira Rios, Marcia S Vieira, Mariana Lemos de Moraes, Otoniel Félix de Souza, Thaís Bastos Stefanello, Christelle Boudry, Ludovic Lahaye

Bibliographic record

VenuePoultry Science · 2025
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicAnimal Nutrition and Physiology
Canadian institutionsCegep de Saint Hyacinthe
FundersConselho Nacional de Desenvolvimento Científico e Tecnológico
KeywordsBroilerBacillus subtilisXylanaseFood scienceNutrientBiologyChemistryBiochemistryBacteriaEnzymeEcology

Abstract

fetched live from OpenAlex

The objective of this experiment was to evaluate the effects of a Bacillus subtilis xylanase on nutrient digestibility, intestinal health, and growth performance of broiler chickens undergoing an intestinal challenge. Nine hundred one-day-old Cobb 500 male chicks were randomly distributed into three dietary treatments with 10 replicates (30 birds/pen) until 42 d. Treatments were: Control (CT) diet, energy reduction (ER) diet, and the ER diet supplemented with B. subtilis endo 1,4-β-xylanase at 100 g/t (ER+xylanase). The ER diets had metabolizable energy reductions as per manufacturer guidelines: 80, 82, 87, and 92 kcal/kg for pre-starter, starter, grower, and finisher, respectively. All birds were challenged with Eimeria spp. on d 1 and Clostridium perfringens on d 12-14. Feed intake, body weight gain (BWG), and feed conversion ratio (FCR) were evaluated weekly. On d 17, the intestinal permeability was determined using fluorescein isothiocyanate-dextran (FITC-d) as marker. On d 21, I See Inside (ISI) methodology assessed intestinal alterations. On days 21 and 42, nutrient digestibility and cecal short-chain fatty acids (SCFA) concentrations were calculated. Data underwent ANOVA or Kruskal-Wallis tests, followed by Fisher or Dunn tests, respectively. From 0 to 42 d, broilers fed the ER+xylanase diet had greater (P = 0.046) BWG than birds fed the ER diet, while FCR showed improvements in CT and ER+xylanase diets. Broilers fed the ER+xylanase diet had improved intestinal integrity with the lowest (P = 0.017) serum FITC-d concentration and highest (P = 0.057) Mucin-2. Pancreas ISI scores were worsened (P = 0.034) in CT and ER diets compared to ER+xylanase diet. Ileal digestibility of DM and energy, and SCFA were greater (P ≤ 0.006) in broilers fed the ER+xylanase diet compared to ER diet. In conclusion, the B. subtilis xylanase supplementation improved nutrient digestibility, enhanced intestinal barrier integrity and increased SCFA concentrations which improved growth performance in broilers undergoing an intestinal challenge. Results indicated that this xylanase was able to compensate the energy reduction in corn-soy diets fed to broiler chickens.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.539
Threshold uncertainty score0.323

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.260
Teacher spread0.237 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations5
Published2025
Admission routes1
Has abstractyes

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