Gaping conditions of the Pectoralis minor (tenders) in commercial broilers: Prevalence, histology, and gene expression
Bibliographic record
Abstract
Gaping is a recently described condition that affects the Pectoralis minor (tender) muscle of broiler chickens, characterized by post-mortem separation of myofiber that leads to meat depreciation and economic losses. In this study, we aimed at understanding prevalence, morphological features, and transcriptomics signatures of this poorly understood myopathy. Between July 2022 and January 2023, a total of 5,180 chicken tenders were collected from 32 flocks across two plants in the USA, handling light (2.7 kg) and heavy (4.1 kg) birds. The prevalence of moderate and severe gaping was 24.8 % and 53.7 %, respectively. The light bird plant had a lower prevalence of moderate gaping (P < 0.001), while the heavy bird plant had a lower prevalence of severe gaping (P < 0.001). Spaghetti meat prevalence from 8,000 fillets was 46.9 % for moderate and 8.3 % for severe cases, with no significant inter-plant differences. Use of peracetic acid treatment at the poultry plants significantly increased the prevalence of severe gaping. Physical and histological features, along with gene expression, were evaluated in 120 samples representative of three gaping severity tiers. Severely gaped tenders showed greater width compared to normal and moderately gaped tenders in both light and heavy birds (P < 0.05). An increase of 1 cm in tender width was associated with a 1.99-fold increase in the odds of classification into a more severe gaping category (95 % CI: 1.15 - 3.46). Affected muscles revealed histological evidence of myodegeneration, inflammation, and lipidosis with fibrosis. For one-unit increase in the myodegeneration score, samples had a 1.75-fold increase in the odds of being classified into a more severe gaping category (95 % CI: 1.37 - 2.23). Gene expression analysis using droplet digital PCR showed differential expression of 19 genes involved in oxidative stress response, cellular signaling, muscle development, and collagen formation between weight groups and myopathy categories. Notably, 21 out of 22 differentially expressed genes showed higher expression in light birds. This study provides the comprehensive description of gaping in broiler chickens and lays a crucial benchmark for assessment of future mitigating strategies.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".