Inactivation of Salmonella and avian pathogens on hatchery eggs using gas phase hydroxyl-radical process vs formaldehyde fumigation: Efficacy, hatching performance and grow-out of Chickens
Bibliographic record
Abstract
Hatcheries have been identified as a significant source of Salmonella within poultry production. Consequently, there is a need for effective egg disinfection methods that can reduce the pathogen burden while preserving the egg integrity and embryo. The metrics for a successful egg disinfection method are typically a reduction in Total Aerobic Count (TAC) while retaining hatching rates. In this study, a gas phase hydroxyl-radical process was validated and verified as a hatchery egg disinfection method. The process is based on applying a hydrogen peroxide mist in combination with ozone gas and UV-C to generate antimicrobial hydroxyl radicals. The treatment (2% hydrogen peroxide, 20 ppm ozone and 19 mJ/cm 2 UV-C; designated as HR) for inactivating Salmonella (serotypes Enteritidis and Typhimurium) inoculated onto eggs could eliminate the pathogen (>5 log CFU/egg reduction) but left residual TAC (1.53 log CFU/egg reduction). Surface sterilization was achieved by a pre-treatment of eggs with the photo-catalyst riboflavin (13.75 mM) followed by 3% hydrogen peroxide delivered at 70°C prior to the hydroxyl-radical treatment (3% hydrogen peroxide, 20 ppm ozone and 114 mJ/cm 2 designated HRS). The surface sterilization of eggs coincided with the removal of the cuticle layer with the HRS treatment but not HR. The cuticle layer was also compromised by formaldehyde treatment. When the different treatments were applied to fertile hatchery eggs (n=50 eggs per treatment group), there was no significant difference in hatchery rate (64-74%), with hatch to fertility being higher for disinfected eggs (89-97%) compared to the non-treated control (80%). The seven-day mortality (0 – 2 birds) and feed conversion ratio (1.59 – 1.75 kg/kg feed) did not significantly differ between the treated vs controls. The HR treatment could eliminate Enterococcus faecium, Escherichia coli (>5 log CFU/egg reduction) although HRS was required to inactivate Pseudomonas aeruginosa (>5 log CFU/egg reduction) and reduce Aspergillus niger spores (3.08±2.25 log CFU reduction). The study has provided treatment options for hatchery egg disinfection and alternative to formaldehyde treatment.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".