Suppression of a Transketolase Mutation Leads to Only Partial Restoration of Symbiosis in <i>Sinorhizobium meliloti</i>
Bibliographic record
Abstract
The interaction between Sinorhizobium meliloti and alfalfa is a well-studied model system for symbiotic establishment between rhizobia and legume plants. Proper utilization of carbon sources has been linked with effective symbiotic establishment in S. meliloti strain Rm1021. Previous work has shown that mutation of the gene tktA, which encodes a transketolase involved in the pentose phosphate pathway, resulted in a strain impaired in many biological functions, including the ability to establish symbiosis with alfalfa. Work with this strain revealed the appearance of suppressor mutations that could partially revert the symbiotic phenotype associated with a tktA mutation. Characterization of these suppressor strains showed that carbon phenotypes associated with a mutation in tktA were no longer present and that the production of succinoglycan was partially restored. Central carbon metabolite pools were observed to be different compared with the wild-type and tktA mutant strains. Multiple independent mutations were identified in the gene SMc02340, a Gnt-type negative regulator, upon sequencing. RT-PCR suggested that SMc02340 acts as a negative regulator on an operon containing the gene tktB, which becomes upregulated when the suppressor mutation is present or SMc02340 is removed. Microscopic analysis revealed a unique symbiotic phenotype. The tktA mutant strain induced root hair curling but could not colonize the apoplastic space. Collectively, the data suggest that the upregulation of tktB can partially bypass some blocks associated with a lesion in tktA, including the colonization of the curled root hair, but cannot fully compensate for the loss of tktA. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".