The PBAP chromatin remodeling complex mediates summer diapause via H3K4me3-driven juvenile hormone regulation in <i>Colaphellus bowringi</i>
Bibliographic record
Abstract
Diapause, a developmental arrest mechanism, helps animals to survive seasonal changes via endocrine regulation. While obligate diapause is genetically programmed, facultative diapause is typically triggered by environmental cues such as photoperiod. In insects, this often leads to reproductive diapause characterized by reduced juvenile hormone (JH) signaling, resulting in ovarian arrest and lipid accumulation. However, the molecular link between photoperiod and hormonal control remains poorly understood. In this study, we investigated the cabbage beetle Colaphellus bowringi as our model system. This species exhibits a photoperiodic response, where short-day (SD) conditions promote reproduction, whereas long-day (LD) conditions induce diapause. Our research identified the PBAP chromatin remodeling complex as a key regulator of LD-induced summer diapause entry. Through RNAi screening of 56 transcriptional regulators that were differentially expressed between SD and LD females, we identified BAP170, a PBAP-specific component, as a key mediator of diapause. Knockdown of bap170 in SD females induced reproductive diapause traits, which were reversed by treatment with methoprene, a JH analog, suggesting that the PBAP complex regulates diapause by influencing JH production. We further demonstrated that the PBAP complex modulates JH biosynthesis via SET1/COMPASS-mediated trimethylation of H3K4. Transcriptome analysis and a second RNAi screen identified calmodulin , a calcium-binding messenger protein gene, as a direct target of PBAP-SET1/COMPASS-H3K4me3 signaling in the corpora allata (CA), the primary source of JH. These findings reveal how the chromatin remodeling machinery translates photoperiod signals into endocrine responses governing seasonal adaptation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".