Somaclonal variation in regenerants from long-term embryonic cultures of <i>Chlorophytum borivilianum</i> obtained from agamospermic seeds of a triploid plant
Why this work is in the frame
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Bibliographic record
Abstract
Summary Chlorophytum borivilianum is an endangered aphrodisiac plant and most of the plants in nature are triploid. Agamospermic seeds of a triploid plant were used to initiate somatic embryogenesis. Plantlets obtained through seedling-derived embryonic callus showed high level of morphological and cytological variation, which increased with the increase in age of cultures. Variegated leaves were also observed occasionally. High level of variation in the leaf size, stomata number and epidermal cell size was observed. Increase in the variation in chromosome number was observed with the increase in age of cultures. Chromosomal variation was least 2n=3x-3 to 2n=3x+3 in 1 to 4 months-old cultures and increased with the age of cultures with 2n=5x-1 to 7x in cultures older than 6 months. Therefore, an alternative method was developed to reduce somaclonal variation in germinated somatic embryos, by using leaf from genetically stable plants (1-4 months-old cultures) as a source of explants for re-initiation of fresh embryonic cultures. RAPD finger printing of plantlets raised by this method revealed very little variation (6.15%). Further improvement in somatic embryogenesis is required towards domestication of the plant using biotechnological method of propagation.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it