Induction profiles and properties of a novel stress-induced peroxidase in <i>Neurospora crassa</i>
Bibliographic record
Abstract
Exposure of Neurospora crassa cells to heat shock and oxidative stress results in the synthesis of several stress-related proteins, including a peroxidase. Northern blot analysis of total RNA revealed a heat-inducible (HI)-peroxidase transcript of ～10 kb, induced in response to heat shock and oxidative stress. The HI peroxidase was isolated from heat-shocked mycelium and purified to near homogeneity, and its properties were examined. Chromatography in size-exclusion matrices yielded an apparent molecular mass of ～116 kDa for the native enzyme, whereas the estimate obtained by SDS-PAGE was 90–95 kDa. Studies of substrate saturation kinetics were conducted using the purified enzyme with ABTS [2,2'-azino-bis (3- ethylbenzthiazole-6-sulfonic acid)] and H2O2 as substrates. The experimentally estimated Km, Vmax, and Kcat values for ABTS were ～36 μM, 5200 nmolmg-1, and 8 s-1, respectively, and those for H2O2 were 44 μM, 6640 nmolmg-1, and 10 s-1. O-dianisidine was a substrate for this enzyme, but guaiacol was not. HI peroxidase was found to be a glycoprotein, stable at temperatures up to 60°C.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".