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Comparing kinetic proofreading and kinetic segregation for T cell receptor activation

2025· article· en· W4409086045 on OpenAlexafffund
Alexander S. Moffett, Kristina A. Ganzinger, Andrew W. Eckford

Bibliographic record

VenuePhysical Review Research · 2025
Typearticle
Languageen
FieldMedicine
TopicCAR-T cell therapy research
Canadian institutionsYork University
FundersNatural Sciences and Engineering Research Council of CanadaAdvanced Research Projects AgencyDefense Advanced Research Projects AgencyOncode InstituteNederlandse Organisatie voor Wetenschappelijk Onderzoek
KeywordsKinetic energyProofreadingBiophysicsChemistryComputational biologyCell biologyComputer scienceBiologyPhysicsBiochemistryEnzymePolymerase

Abstract

fetched live from OpenAlex

The T cell receptor (TCR) is a key component of the adaptive immune system, recognizing foreign antigens (ligands) and triggering an immune response. To explain the high sensitivity and selectivity of the TCR in discriminating “self” from “non-self” ligands, most models evoke kinetic proofreading (KP) schemes, however it is unclear how competing models used for TCR triggering, such as the kinetic segregation (KS) model, influence KP performance. In this paper, we consider two different TCR triggering models and their influence on subsequent KP-based ligand discrimination by the TCR: a classic conformational change model (CC-KP), where ligand-TCR binding is strictly required for activation, and the kinetic segregation model (KS-KP), where only residence of the TCR within a close contact devoid of kinases is required for its activation. Building on previous work, our computational model permits a head-to-head comparison of these models . While we find that both models can be used to explain the probability of TCR activation across much of the parameter space, we find biologically important regions in the parameter space where significant differences in performance can be expected. Furthermore, we show that the available experimental evidence may favor the KS-KP model over CC-KP. Our results may be used to motivate and guide future experiments to determine accurate mathematical models of TCR function.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.373
Threshold uncertainty score0.451

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.138
GPT teacher head0.468
Teacher spread0.331 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2025
Admission routes2
Has abstractyes

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