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Record W4409172304 · doi:10.1101/2025.03.31.646411

Evolutionary Adaptations of TRPA1 Thermosensitivity and Skin Thermoregulation in Vertebrates

2025· preprint· en· W4409172304 on OpenAlexafffund
Gabriel E. Bertolesi, Neda Heshami, Sarah McFarlane

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldMedicine
TopicThermoregulation and physiological responses
Canadian institutionsUniversity of Calgary
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsThermoregulationSkin temperatureBiologyEvolutionary biologyZoologyEcologyMedicineBiomedical engineering

Abstract

fetched live from OpenAlex

Abstract Altering skin color and reflectance is crucial for temperature regulation in poikilothermic vertebrates, while less so in homeotherms like birds and mammals, which evolved feathers, fur, and other insulation for endothermy. Heat-sensing in vertebrates relies primarily on Transient Receptor Potential (TRP) channels, with certain channels (TRPA1) shifting thermosensitivity over evolution and others retaining heat sensitivity (TRPV1). Exploration of a role for TRP channels in skin physiology has largely focused on human pigmentation and overlooked the evolution of different thermoregulatory structures in the integument of distinct vertebrates. For instance, colour/reflector pigment cells in ectotherms, fur and feathers in endotherms, hairless skin in hominids, and blubber in marine mammals. Therefore, we investigated whether a TRP channel mediates skin darkening induced by heat in the ectotherm Xenopus laevis and then explored the evolution of TRPA1 thermal sensitivity and its link with skin physiology. We find Trpa1 mediates heat-induced melanosome dispersion, darkening skin under warmer conditions. In contrast, TRPA1 is known to mediate cold sensation in rodents and UV-induced tanning in humans, leading us to investigate the co-evolution of TRPA1 and skin thermoregulation. Our findings reveal TRPA1 is a heat sensor in ectotherms with uncovered integuments. In mammals, we suggest TRPA1 was thermally insensitive in Euarchontoglires but became cold-sensitive in several rodent lineages. TRPA1 shows reduced selection pressure for thermosensitivity in aquatic mammals (manatees, whales) that depend on blubber for insulation as compared to their terrestrial relatives. These findings emphasize adaptive evolution of TRPA1 in vertebrates, linking thermal sensitivity to the evolution of skin physiology.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Theoretical or conceptual · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.249
Teacher spread0.231 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designTheoretical or conceptual
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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