Ethylene sensitivity assay in medicinal and fiber-type <i>Cannabis</i> seedlings reveals a triple-response-like phenotype
Bibliographic record
Abstract
This study examines the conservation of ethylene response mechanisms in cannabis seedlings, expanding on evidence of ethylene’s influence in mature plants, particularly sexual plasticity. Using hemp and drug-type seedlings, we observed a unique triple-response-like phenotype under dark conditions with ethephon treatment, characterized by hypocotyl and radicle shortening alongside hypocotyl thickening. Employing a novel ethephon-based assay, this research circumvents the challenges of gaseous ethylene, enabling more accessible investigation of ethylene sensitivity in early development. Results showed high responsiveness to low ethephon concentrations (125–500 mg/L), unlike prior findings in mature plants, while silver thiosulfate (1–3 mmol/L) consistently reversed ethylene effects, supporting the conservation of ethylene signaling pathways across genetic backgrounds. Interestingly, the absence of an exaggerated apical hook in treated seedlings suggests unique regulatory adjustments in achene-bearing plants. This study advances our understanding of ethylene signaling in early cannabis development and provides a foundation for future research on ethylene’s role in developmental processes, including sexual plasticity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".