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Record W4409601260 · doi:10.1016/j.trac.2025.118273

Assay formats and target recognition strategies in lateral flow assays for the detection of mycotoxins

2025· article· en· W4409601260 on OpenAlexaff
Monika Conrad, Maria C. DeRosa

Bibliographic record

VenueTrAC Trends in Analytical Chemistry · 2025
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicMycotoxins in Agriculture and Food
Canadian institutionsCarleton University
Fundersnot available
KeywordsMycotoxinArtificial intelligenceComputer scienceComputational biologyPattern recognition (psychology)BiologyBiotechnology

Abstract

fetched live from OpenAlex

Lateral flow assays for mycotoxins are important analytical tools because of the toxicity of mycotoxins. As mycotoxins can occur in beverages, food, and feed, there are many routes of exposure that lead to their chronic toxicity. Therefore, a monitoring method is needed that offers rapidity, reliability, specificity, and easy on-site operation. Lateral flow assays can offer quick, simple, accurate, and convenient testing. That is why, they are the perfect tool to meet this need. In recent years, many research papers have been published discussing lateral flow assays for mycotoxins. This literature review analyses the published literature of the last ten years with respect to different strategies for the detection of mycotoxins with a focus on assay formats and test strip set-up. Novel formats based on aptamers as recognition elements instead of the most common antibodies are presented. Technological differences are highlighted, and limitations of the used methods are discussed. • Review of lateral flow assays for mycotoxin detection. • Focus on different detection strategies and assay set-up. • Summary of design-strategies for ‘turn-on’ lateral flow assays and multiplexing. • Highlight on aptamer-based lateral flow assays. • Guidance on how to achieve low limits of detection.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.549
Threshold uncertainty score0.158

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.254
Teacher spread0.236 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations10
Published2025
Admission routes1
Has abstractyes

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