Arabidopsis ecotype screening reveals novel sources of clubroot resistance and insights into resistance inheritance
Bibliographic record
Abstract
ABSTRACT Clubroot, caused by Plasmodiophora brassicae , poses a persistent threat to Brassicaceae crops, particularly in regions where resistant cultivars are under strong selection pressure. To identify new sources of resistance and better understand the underlying genetic mechanisms, we evaluated 60 Arabidopsis thaliana ecotypes against the highly virulent Canadian pathotype 3A. Using stringent phenotyping criteria, pathogen DNA quantification, and survival analysis, we identified eight resistant ecotypes, including two novel sources, Marce-1 and DraII-6. DraII-6 exhibited exceptionally low disease symptoms and a high survival rate. While the resistance gene RPB1/WeiTsing was present in most ecotypes, its expression in DraII-6 was significantly elevated at early infection stages, suggesting a potential role in pathogen suppression. However, genetic analysis of F1 and F2 progeny from a DraII-6 × Col-0 cross revealed a recessive resistance pattern, supporting the hypothesis that RPB1 alone may not be sufficient to confer resistance to clubroot in DraII-6. Our findings highlight the complexity of clubroot resistance and the need for further research into gene regulation and resistance networks beyond RPB1, particularly in the context of translating Arabidopsis-based insights to Brassica crops. GRAPHICAL ABSTRACT Natural variability of Arabidopsis thaliana ecotypes in response to the Canadian clubroot pathotype 3A. The abstract also celebrates the first author, Melaine Gonzalez-Garcia, who is submitting this manuscript just one week before welcoming her first child.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".