Using synchrotron FTIR spectromicroscopy to assess physiological and metabolic changes in photosynthetic mutants of <i>Chlamydomonas reinhardtii</i>
Bibliographic record
Abstract
We utilized synchrotron Fourier-transform infrared spectromicroscopy to study photosynthetic mutants of the unicellular green algae Chlamydomonas reinhardtii. We were able to observe direct and knock-on effects of multiple mutations on the physiology and metabolism of individual living cells, and cleanly differentiate the spectra of cell lines that differed by a single point mutation. Phenotypic changes (direct or knock-on) included altered composition and relative abundance of storage and structural lipids, membrane compositions, relative abundance of photosynthetic and photoprotective pigments, abundance and structure of Photosystems I and II, and secondary structure of protein pools. In one mutant, the mutations combined to trigger cellular responses normally seen as cellular adaptation to ongoing high light stress, even under normal light conditions. We were further able to observe that one cell line had phenotypic changes indicative of knock-on physiological beyond those predicted by the point mutation itself.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".