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Record W4409878928 · doi:10.1101/2025.04.28.649888

A real-time detection and non-destructive warning method for zebrafish body surface anomalies based on improved YOLOv8 framework

2025· preprint· en· W4409878928 on OpenAlexaff
Danying Cao, Yang Hong, Yingyin Cheng, Wanting Zhang, Mijuan Shi, Xiao-Qin Xia

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldComputer Science
TopicDigital Imaging for Blood Diseases
Canadian institutionsMinistry of Agriculture
FundersNational Key Research and Development Program of China
KeywordsZebrafishWarning systemBody surfaceSurface (topology)Computer scienceReal-time computingArtificial intelligenceComputer visionData miningBiologyMathematicsGeometryTelecommunicationsBiochemistry

Abstract

fetched live from OpenAlex

Abstract The detection of anomalies on fish surfaces is of critical importance for assessing fish health status, preventing fish disease outbreaks, predicting changes in water quality, enhancing fish welfare. The zebrafish ( Danio rerio ), a key model organism, has been increasingly utilized in various fields, including medicine, genetics and environmental toxicology. This has led to a corresponding increase in demand for intelligent management and detection systems. However, traditional methods of fish disease detection may have irreversible effects on fish, particularly small species, and often fail to meet the precision, non-destructive warning, and real-time requirements for zebrafish detection. To address this issue, this study proposes a novel method based on the YOLOv8 framework, designated ESC-YOLOv8-seg. This method significantly enhances the precision and speed of detecting surface abnormalities on small fish in complex settings by integrating the EMA the SPPELAN and C2f-Faster modules, and adding a detection head tailored for small targets. Furthermore, the integration of positional information and surface features enables the method to achieve real-time monitoring and non-destructive early warning of fish surface abnormalities. The proposed method enhances precision in small target detection and achieves high accuracy in discerning subtle differences among detection targets. In real aquaculture settings, it can reach an average speed of 106 FPS with a detection accuracy of 98%. Although this study has been designed to meet the specific needs of zebrafish scientific research, it is highly generalisable and can be applied to the real-time detection of underwater surface abnormalities in a range of fish species in aquaculture.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Scholarly communication
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: none
Teacher disagreement score0.602
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0010.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.237
Teacher spread0.230 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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