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Record W4410030081 · doi:10.1016/j.ohx.2025.e00653

Leveraging flexible pipette-based tool changes to transform liquid handling systems into dual-function sample preparation and imaging platforms

2025· article· en· W4410030081 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueHardwareX · 2025
Typearticle
Languageen
FieldEngineering
TopicMicrofluidic and Capillary Electrophoresis Applications
Canadian institutionsUniversity of New BrunswickStructural Genomics ConsortiumUniversity of Toronto
FundersNatural Sciences and Engineering Research Council of CanadaCanada First Research Excellence FundUniversity of Toronto
KeywordsPipetteSample (material)Computer scienceDual (grammatical number)Function (biology)Computer hardwareBiomedical engineeringNanotechnologyMaterials scienceChromatographyChemistryEngineering

Abstract

fetched live from OpenAlex

In soft materials synthesis, rapid self-assembly and poor mechanical strength often limit the applicability of experimental characterization techniques. This limitation arises because transferring these materials to a suitable imaging platform is either too slow to capture the process of interest or impossible to safely transfer from the synthesis vessel to the characterization. In addition, the variable nature of these materials requires many experiments to understand the underlying structure-property relationships that govern these materials. In this work we present a new hardware platform that integrates simultaneous pipetting and in-situ imaging using the Opentron OT-2 liquid handling robot. A 3D printed adapter features two cylindrical openings, one containing the pipette tip to gantry adapter, and the other a USB camera. When the gantry picks up the pipette tip, the entire apparatus is lifted, allowing the camera to be used. This system enables real-time monitoring and characterization of dynamic processes, such as hydrogel crosslinking, without manual intervention. We used this system to characterize ionically crosslinked hydrogels, and monitored their properties over time, in a high-throughput and combinatorial manner. Although hydrogels were used as a proof-of-concept, this platform has broader applications in materials research, including crystallization dynamics, polymerization kinetics, and drug delivery system development.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.654
Threshold uncertainty score0.749

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.222
Teacher spread0.214 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it