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Record W4410283591 · doi:10.1242/jeb.250501

Unlocking the mystery of mountain horned lizard reproduction

2025· article· en· W4410283591 on OpenAlexaff
Alexandra N. Schoen

Bibliographic record

VenueJournal of Experimental Biology · 2025
Typearticle
Languageen
FieldEnvironmental Science
TopicWildlife Ecology and Conservation
Canadian institutionsUniversity of Winnipeg
Fundersnot available
KeywordsLizardReproductionGeographyEcologyBiology

Abstract

fetched live from OpenAlex

It is easy to imagine how living things communicate with each other. However, what about communication inside our own body? We can't necessarily see or hear what is going on inside; nevertheless, hormones are constantly sharing information throughout the body, speeding between organs to keep everything running smoothly. Hormones are important for lots of different processes, such as sleep and reproductive cycles. In reproduction, hormones can control when some animals breed, get pregnant and give birth. Scientists can figure out when these reproductive events might happen by measuring the reproductive hormones in an animal's blood. This is particularly important for endangered species, such as the mountain horned lizard (Phrynosoma orbiculare), found in the Parque de la Ciencia Sierra Morelos, Mexico. These lizards are vulnerable to future habitat damage, so knowing more about their reproduction is critical in helping save them from extinction. Laura Elena Hernández-Hernández at the Universidad Autónoma del Estado de México, Mexico, worked with colleagues from Mexico and the USA to determine how the lizards’ hormones changed throughout the year and how these hormones were related to temperature and day length.The researchers collected blood samples from the tails of 31 female horned lizards every month for 12 months, and then measured three hormones related to reproduction (progesterone, estradiol and testosterone). The researchers also felt the stomach of the lizards to see whether they were pregnant. Hernández-Hernández and colleagues found that progesterone increased during the horned lizards’ pregnancy period between September and January. The lizards remained pregnant until April, but their progesterone levels started to decrease in February and kept decreasing until they gave birth. Hernández-Hernández suggests that progesterone helps keep pregnancy going ­– as it does in other animals – and is more important at the beginning, but other hormones might be more important towards the end of the lizard’s pregnancy.One of these other hormones – estradiol – showed the opposite pattern to progesterone and was higher towards the end of the lizard’s pregnancy, but decreased in the mother lizards after birth. Estrodiol is important for egg growth in other reptiles and the researchers believe that, in horned lizards, estradiol helps in moving nutrients into eggs, as well as helping female lizards become ready to breed again. Additionally, the team speculated that the pattern between progesterone and estradiol levels shows that these hormones might interact with each other throughout the year.The final hormone that the researchers tested was testosterone. The testosterone in the lizards’ blood was higher during the breeding season but decreased in later pregnancy. Hernández-Hernández and colleagues also found that testosterone quickly increased before birth and remained high through the next breeding season. The researchers suggest that, like estradiol, testosterone might help female lizards get ready to breed. Additionally, the scientists compared the hormone levels with environmental conditions (temperature, rainfall and daylight hours) to see whether they were related. The team found that, as the yearly temperature increased, progesterone decreased while estradiol increased. The warmest temperatures occurred during the breeding season, meaning that the lizards had low progesterone and high estradiol levels. These patterns were also the same for day length, where longer days were during the breeding season, which makes sense as temperature and day length are connected (longer days during warmer summer months).Ultimately, Hernández-Hernández and colleagues showed that blood samples and hormones can be used to figure out when the lizards are pregnant, breeding or preparing nutrients for the next year's offspring. This is very important in a species that is at risk from habitat damage and could help scientists learn about reproduction in other endangered reptile species.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.004
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.007
Threshold uncertainty score0.015

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0020.004
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.000
Science and technology studies0.0020.008
Scholarly communication0.0030.005
Open science0.0010.002
Research integrity0.0020.005
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.278
Teacher spread0.264 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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