Functional characterization of the N assimilation pathways in the mycelium of <i>Laccaria bicolor</i> and the ectomycorrhizal symbiosis
Bibliographic record
Abstract
Ectomycorrhizal (ECM) fungi contribute to N, Pi, and water uptake in trees while obtaining carbohydrates from their host plants. However, the molecular mechanisms underlying N assimilation during ECM symbiosis remain unclear. In this study, we used RNA interference (RNAi) to silence the expression of genes encoding glutamine synthetase (GS) and NADP-glutamate dehydrogenase (GDH), which are key enzymes involved in N assimilation in the ectomycorrhizal basidiomycete, Laccaria bicolor. LbGS and LbGDH RNAi strains exhibited significantly reduced mycelial growth when cultivated with various inorganic N sources. Compared with the wild-type mycelium, the RNAi strains demonstrated a reduced formation rate of ECM rootlets, indicating the essential role of these 2 enzymes in the establishment of symbiosis. Transcriptomic analysis revealed that silencing of LbGS and LbGDH also altered the expression of other genes involved in N metabolism in ECM rootlets. 15N and 13C tracer experiments demonstrated that LbGS silencing affects carbon exchange in ECM roots. Our findings have established that both GS and NADP-GDH pathways play crucial roles in N assimilation in free-living mycelia and ECM roots, although the GS/GOGAT pathway appears to be predominant.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".