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Record W4410458550 · doi:10.1093/jimmun/vkaf105

Ly49G, but not Ly49C/I, is dispensable for diverse antigen-specific memory NK cell responses in H-2d and H-2b mice

2025· article· en· W4410458550 on OpenAlexafffund
Gayani S. Gamage, Daniel Medina-Luna, Michal Scur, Sayanti Dey, Andrew Wight, Zhongjun Dong, Brendon Parsons, Mir Munir A. Rahim, Andrew P. Makrigiannis

Bibliographic record

VenueThe Journal of Immunology · 2025
Typearticle
Languageen
FieldImmunology and Microbiology
TopicImmune Cell Function and Interaction
Canadian institutionsUniversity of AlbertaUniversity of WindsorBeatrice Hunter Cancer Research InstituteDalhousie University
FundersCanadian Institutes of Health Research
KeywordsAntigenCell biologyBiologyImmunology

Abstract

fetched live from OpenAlex

Immunological memory is a hallmark of the adaptive immune system. However, considerable evidence indicates that the natural killer (NK) cell subset of innate lymphoid cells also mediates specific memory responses to diverse antigens, including peptides. Even though the existence of NK cell memory is established, the mechanism behind NK cell adaptive responses is yet to be elucidated. Previously, we observed that the Ly49 family of class-I MHC receptors in mice are critical for the formation of adaptive NK cell memory responses. To define the nature of Ly49 involvement in NK cell memory responses, we investigated the contribution of individual Ly49 receptors and their defined class-I MHC ligands. We determined that the Ly49 requirement for the generation of NK memory responses is not uniform. Specifically, Ly49C and/or Ly49I proteins are indispensable for the adaptive NK cell responses as assessed by contact hypersensitivity recall responses to haptens and peptides, in H-2b and H-2d MHC backgrounds. In contrast, the highly expressed inhibitory receptor, Ly49G, did not appear to play any role in NK cell memory responses as determined using antibody-mediated subset depletion and gene-deficient mouse models, even in strains containing known ligands for Ly49G. These findings point to a unique role for Ly49C/I in adaptive NK cell antigen recognition and provide further insight into the mechanism behind adaptive NK cell responses.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0010.000
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0030.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.249
Teacher spread0.231 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2025
Admission routes2
Has abstractyes

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