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Record W4410729474 · doi:10.1101/2025.05.20.655047

Fat-rich diet reprograms intrapulmonary neutrophils to boost tissue-specific antitumor immunity

2025· preprint· en· W4410729474 on OpenAlexfundno aff
Yanling Wang, Jinjing Zhang, Minghao Yin, Tao Wang, Lu Wang, Yi Miao, Chia‐Wei Chang, Yuanyuan Liu, Ziyang Huang, He Xu, Lei Wang, Yushi Yao

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldImmunology and Microbiology
TopicImmune cells in cancer
Canadian institutionsnot available
FundersNatural Science Foundation of Zhejiang ProvinceFundamental Research Funds for the Central UniversitiesZhejiang UniversityChina Postdoctoral Science FoundationNational Natural Science Foundation of ChinaMcMaster University
KeywordsImmunityImmunologyMedicineBiologyImmune system

Abstract

fetched live from OpenAlex

Abstract Neutrophils adapt to tissue-specific signals and exert innate defense functions against infections and malignancies. Fat-rich diet (FRD), such as high-fat diet (HFD) and ketogenic diet (KD), has complex impacts on immunity. However, whether and how FRD shapes tissue-specific functions of neutrophils remain unclear. Here we show that both isocaloric HFD- and KD-fed mice demonstrate enhanced neutrophil-mediated pulmonary tumor resistance than chow diet-fed mice. Intrapulmonary but not systemic neutrophils in FRD-fed mice bear enhanced potential of reactive oxygen species (ROS) production and ROS-dependent tumor cytotoxicity. Mechanistically, FRD-induced increased serum saturated fatty acids and cholesterol stimulate lung vascular endothelial cells (LVECs), which reprogram intrapulmonary neutrophils via contact- and intercellular adhesion molecule-1 (ICAM-1)-dependent mechanisms. Analysis on human lung single-cell RNA sequencing data showed that intensified cell adhesion and priming signals from human LVECs are associated with enhanced antitumor functions in intrapulmonary neutrophils. Our findings highlight the roles of dietary fats in shaping neutrophil functions in a tissue-specific manner. Dietary intervention targeting tissue-specific reprogramming of neutrophils therefore represents a potential strategy against malignancies in the lungs.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.239
Teacher spread0.224 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicImmune cells in cancerFrench-language works237,207