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Record W4410808471 · doi:10.1371/journal.pcbi.1013115

RETINA: Reconstruction-based pre-trained enhanced TransUNet for electron microscopy segmentation on the CEM500K dataset

2025· article· en· W4410808471 on OpenAlexafffund
Xing Cheng, Ronald Xie, Gary D. Bader

Bibliographic record

VenuePLoS Computational Biology · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCell Image Analysis Techniques
Canadian institutionsSinai Health SystemPrincess Margaret Cancer CentreLunenfeld-Tanenbaum Research InstituteUniversity of TorontoUniversity Health NetworkCanadian Institute for Advanced Research
FundersCanadian Institutes of Health Research
KeywordsArtificial intelligenceSegmentationComputer scienceConvolutional neural networkPattern recognition (psychology)Image segmentationDeep learningArtificial neural networkComputer visionScale-space segmentationFeature extraction

Abstract

fetched live from OpenAlex

Electron microscopy (EM) has revolutionized our understanding of cellular structures at the nanoscale. Accurate image segmentation is required for analyzing EM images. While manual segmentation is reliable, it is labor-intensive, incentivizing the development of automated segmentation methods. Although deep learning-based segmentation has demonstrated expert-level performance, it lacks generalizable performance across diverse EM datasets. Current approaches usually use either convolutional or transformer-based neural networks for image feature extraction. We developed the RETINA method, which combines pre-training on the large, unlabeled CEM500K EM image dataset with a hybrid neural-network model architecture that integrates both local (convolutional layer) and global (transformer layer) image processing to learn from manual image annotations. RETINA outperformed existing models on cellular structure segmentation on five public EM datasets. This improvement works toward automated cellular structure segmentation for the EM community.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Simulation or modeling · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.018
Threshold uncertainty score0.036

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.003
Meta-epidemiology (narrow)0.0030.001
Meta-epidemiology (broad)0.0010.002
Bibliometrics0.0020.002
Science and technology studies0.0010.001
Scholarly communication0.0020.001
Open science0.0030.001
Research integrity0.0020.002
Insufficient payload (model declined to judge)0.0060.007

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.311
Teacher spread0.303 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designSimulation or modeling
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations6
Published2025
Admission routes2
Has abstractyes

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