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Record W4410811958 · doi:10.1093/biomethods/bpaf041

KD_MultiSucc: incorporating multi-teacher knowledge distillation and word embeddings for cross-species prediction of protein succinylation sites

2025· article· en· W4410811958 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueBiology Methods and Protocols · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMachine Learning in Bioinformatics
Canadian institutionsArtificial Intelligence in Medicine (Canada)
Fundersnot available
KeywordsSuccinylationComputer scienceComputational biologyEmbeddingArtificial intelligenceBiological systemChemistryLysineBiologyBiochemistry

Abstract

fetched live from OpenAlex

Protein succinylation is a vital post-translational modification (PTM) that involves the covalent attachment of a succinyl group (-CO-CH2-CH2-CO-) to the lysine residue of a protein molecule. The mechanism underlying the succinylation process plays a critical role in regulating protein structure, stability, and function, contributing to various biological processes, including metabolism, gene expression, and signal transduction. Succinylation has also been associated with numerous diseases, such as cancer, neurodegenerative disorders, and metabolic syndromes. Due to its important roles, the accurate prediction of succinylation sites is essential for a comprehensive understanding of the mechanisms underlying succinylation. Although research on the identification of protein succinylation sites has been increasing, experimental methods remain time-consuming and costly, underscoring the need for efficient computational approaches. In this study, we present KD_MultiSucc, a model for cross-species prediction of succinylation sites using Multi-Teacher Knowledge Distillation and Word Embedding. The proposed method leverages the strengths of both Knowledge Distillation and Word Embedding techniques to reduce computational complexity while maintaining high accuracy in predicting protein succinylation sites across species. Experimental results demonstrate that the proposed predictor outperforms existing predictors, providing a valuable contribution to PTM research and biomedical applications. To assist readers and researchers, the codes and resources related to this work have been made freely accessible on GitHub at https://github.com/nuinvtnu/KD_MultiSucc/.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.269
Threshold uncertainty score0.477

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.053
GPT teacher head0.461
Teacher spread0.408 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it