Enhanced Resistance to Verticillium dahliae in Transgenic Strawberry Plants Expressing a Lycopersicon chilense Chitinase Gene
Bibliographic record
Abstract
A chitinase gene ( pcht28 ), isolated from Lycopersicon chilense , was transferred into `Joliette' strawberry using a stipule regeneration method and Agrobacterium -mediated gene transfer technique. Stipules showed a high rate of shoot regeneration (>90%) through direct or indirect organogenesis. Stipules were cocultured with a transformation plasmid in which pcht28 was under the control of the CAMV 35S promoter. A high tendency in production of chimaeric shoots was observed. Shoots which did not show any sign of bleaching after several subcultures in kanamycin containing medium were considered as stable transformants. These shoots were successfully rooted in the presence of 50 μg·mL -1 kanamycin. Transgenic nature of the plants was confirmed by PCR as well as Southern blot analysis. Constitutive expression of the chitinase gene was demonstrated by Northern analysis. In growth chamber studies, the transgenic strawberry plants which expressed pcht28 had significantly higher resistance to Verticillium dahliae as compared to nontransgenic controls. These results demonstrate that pcht28 plays a role in defense against this fungal disease in strawberry.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".