Genome-wide identification of SmJAZ gene family in eggplant and functional mechanism analysis of SmJAZ9 modulating high temperature and darkness stress-regulated anthocyanin biosynthesis
Bibliographic record
Abstract
The biosynthesis of anthocyanin in plants is regulated by temperature, light, jasmonic acid (JA), among others. JASMONATE ZIM-DOMAIN (JAZ) proteins are critical repressors of the jasmonic acid (JA) pathway, the role of JAZ proteins on anthocyanin biosynthesis under high-temperature or dark conditions remains unexplored. Here, we identified 16 <italic>SmJAZ</italic> genes in eggplant and characterized their evolutionary relationships, gene structures, and high-temperature (HT) and darkness stress-responsive expression patterns. Both the HT and darkness treatments significantly inhibit anthocyanin biosynthesis in eggplant peel. Correlation analysis revealed that the <italic>SmJAZ9</italic> expression strongly correlated with anthocyanin biosynthesis-related genes under HT and darkness conditions. Transient expression assay in eggplant peels demonstrated that nuclear-localized SmJAZ9 is a positive regulator, enhancing anthocyanin biosynthesis via interaction with the transcription factor SmMYB113, thereby promoting <italic>SmCHS</italic> and <italic>SmDFR</italic> transcription. Additionally, SmJAZ9 interacted with SmMYC2 but not SmCOI1, suggesting a JA signaling-independent regulatory mechanism under HT and darkness conditions. This study revealed a novel JAZ-mediated regulatory module integrating JA signaling, environmental stress responses, and anthocyanin biosynthesis, providing potential targets for improving eggplant fruit quality.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".