Molecular Characterization of Trehalose-6-Phosphate Synthetase 2 in Nosema bombycis
Bibliographic record
Abstract
Abstract Pebrine caused by Nosema bombycis infection is one of the most threatening diseases to sericulture. Trehalose-6-phosphate synthetase is a key rate-limiting enzyme in the trehalose metabolism pathway. N. bombycis trehalose phosphate synthetase 2 (NbTPS2) is a subcellular unit of N. bombycis trehalose synthase gene complex. Here, NbTPS2 gene was amplified, sequenced and expressed in E. coli BL21 strain. The result showed that NbTPS2 gene contains an open reading frame with length of 1371 bp and encoded a protein with molecular mass of approximately 57.87 kDa. His pull-down result showed that 66 N. bombycis proteins and 359 silkworm proteins may be directly or indirectly interacted with NbTPS2. Molecular phylogeny analysis result showed that NbTPS2 gene was conservative in different microsporidia. In addition, the highest relative transcription level of NbTPS2 at 6 h after infection suggesting that NbTPS2 plays an important role in the early infection stage (possibly germination stage) for N. bombycis. These results revealed the molecular characteristic of NbTPS2, laying the molecular foundation for the subsequent functional study of NbTPS2.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".