The assembly of the membrane arm of mitochondrial complex I follows evolutionarily distinct routes
Bibliographic record
Abstract
Abstract Complex I is the largest enzyme of the mitochondrial oxidative phosphorylation system. It is composed of core subunits that are conserved across bacteria and eukaryotes and accessory subunits that are lineage-specific. Consequently, the assembly of complex I likely requires lineage-specific assembly machineries. Complex I assembly has been mainly studied in mammals. It follows a modular pathway in which modules are assembled independently and then associated together to form the mature complex. Here, we identify and characterize PAF1 as an assembly factor for the membrane arm of complex I in plants. We show that this protein plays a role in stabilizing a module of the membrane arm prior to its association with the rest of the complex. PAF1 was likely present in the last eukaryotic common ancestor and has been lost in mammals in which the assembly of the membrane arm involves assembly factors not conserved in plants. Altogether, we provide molecular evidence that the assembly of the membrane arm of complex I can be performed by two different machineries.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".