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Record W4411274120 · doi:10.1016/j.jbc.2025.109781

Abstract 2507 Finding an RNA Aptamer Against SOD-1 to be Used as a Therapeutic for ALS Patients

2025· article· en· W4411274120 on OpenAlexaboutno aff
Avery Matthews, Gwendolyn M. Stovall

Bibliographic record

VenueJournal of Biological Chemistry · 2025
Typearticle
Languageen
FieldMedicine
TopicAmyotrophic Lateral Sclerosis Research
Canadian institutionsnot available
Fundersnot available
KeywordsAptamerRNAChemistryComputational biologyBiochemistryMolecular biologyBiologyGene

Abstract

fetched live from OpenAlex

The potential impact of early and efficient detection of biological threats in safeguarding public health and national security highlights the importance of reliable bio-threat detectors.Such detectors could provide a critical line of defence against potential bioterrorism, emerging infectious diseases, and environmental hazards.Performing the initial stages of developing sensors to recognize infectious viruses is challenging when high-safety level environments are required due to the toxicity of the viral targets.Although virus-like particles can be used safely, they are not available for all viral biothreats and can be difficult to produce.Therefore, we are developing a structural imitation of a virus that can be readily produced in any chemistry or molecular biology laboratory and be used to test the efficiency of biosensors during the early assembly and testing stages with no associated health hazards.The design involves attaching a target protein to magnetic or other beads of dimensions similar to the target viruses.The average amount of protein per bead can be quantified using the same detection molecule (e.g.antibodies, aptamers) to be integrated in the sensor.For our development of aptasensors, we are quantifying with a 32P-labeled aptamer using a filter capture assay.Validation of the "proxy" viruses also involves testing them in the aptasensor, which is performed under BSL1 conditions and maintains personnel safety.Financial support was provided by the Department of Homeland Security (DHS) (Cooperative Agreement: 20CWDARI00033).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.012
Threshold uncertainty score0.039

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0120.004

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.108
GPT teacher head0.386
Teacher spread0.278 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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Same venueJournal of Biological Chemistry→Same topicAmyotrophic Lateral Sclerosis Research→French-language works237,207→