Protein corona composition modulates uptake of polymeric micelles by colorectal cancer cells
Bibliographic record
Abstract
-poly(α-benzyl carboxylate-ε-caprolactone) (PEO-PBCL) copolymers with varying degrees of polymerization after incubation in human plasma, and explores their relationship with cellular uptake by colorectal cancer cells. Traceable block copolymers were synthesized, self-assembled into PMs (44-99 nm, slightly negative zeta potentials), and characterized. Protein coronas were formed by incubating PMs with human plasma; protein-coated micelles were separated and analyzed. Uptake of selected PMs, with and without human plasma pre-incubation, by colorectal cancer cells was assessed. PEO-PCL micelles exhibited higher cellular uptake than PEO-PBCL micelles. Human plasma significantly reduced the uptake of PEO-PCL micelles, while PEO-PBCL micelles' uptake remained low. Proteomic analysis identified 23 distinct proteins among the combined top 20 most abundant proteins from each PM corona, with 18 common across all micelle types. In the top 10 proteins, PEO-PCL micelles shared an identical profile, whereas PEO-PBCL micelles had two unique proteins not present in PEO-PCL coronas. Protein corona composition in both PMs was shown to influence their cellular uptake behavior.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".